Effect of plant growth regulator on tissue culture media for trigger of Kepok banana
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Author |
Sugiarto, Rose Novita Sari Handoko and Tri Suyono
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e-ISSN |
1819-6608 |
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On Pages
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2059-2066
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Volume No. |
17
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Issue No. |
24
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Issue Date |
January 29, 2023 |
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DOI |
https://doi.org/10.59018/122210
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Keywords |
musa acuminata x musa balbisiana, growth regulators, tissue culture.
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Abstract
Kepok banana (Musa acuminata x Musa balbisiana) can be grown in a wide area of Indonesia and is still
cultivated regularly in rural areas. The need for commercial-quality of Kepok banana seedlings is still an obstacle to
supporting export success. Seed propagation using micropropagation techniques is an alternative to be developed in order
to meet the needs of appropriate, high-quality, and potential seeds for high productivity. The use of growth regulators and
suitable growth media will promote the success of in vitro culture. The method used is the media Murashige-Skoog (MS).
The design used was completely randomized and arranged by factorial. Factor 1 is a type of growth regulator with a
concentration of 2 mg/L. Factor I is Z1 = Gibberellins; Z2 = IAA; and Z3 = Auxin. Factor II is 50 mg of the growth
medium, namely: M1 = Agar medium; M2= Potato medium; M3 = Medium with coconut; M4 = Media Aloe vera. Data
analysis used F 5% test and 5% BNT test. The use of a gibberellin growth regulator showed a better shoot formation time
of 8 days, the number of shoots was 13.24 seeds, the length of the shoot was 1.25 cm, the root length was 1.52 cm, and the
number of shoots was 77.48%. The growing medium using potatoes and young coconut showed a root length of 0.43 cm
and a root diameter of 0.34 mm. Young coconut media showed better results in the growth of the number of plantlet shoots
as much as 12.53%. The function of growth regulators, gibberellins, IAA, and auxin gave a rapid response to plantlet
meristematic changes and shoot formation. While the use of agar, potato, young coconut, and aloe vera growing media
gave a slow response to plantlet meristematic changes and the formation of Kepok banana shoots.
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