ARPN Journal of Agricultural and Biological Science       ISSN 1990-6145
   
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ARPN Journal of Agricultural and Biological Science        November 2013 | Vol. 8 No. 11
 
Title:

The effect of legumes and non-legumes biomass toward N mineralization on degradated soils in lampung, Indonesia

Author (s):

Andy Wijanarko, Benito Heru Purwanto, Dja'far Shiddieq and Didik Indradewa

Abstract:

This research aims to study the kinetics of N mineralization by using double-pool kinetics models on degradated soils in the cultivation of monoculture cassava as a result of providing organic matter of legume and non-legume. Incubation experiments were conducted in the laboratory at a constant temperature. The treatment was by giving non-legume and legume biomass on Ultisol in which cassava has been planted there with different lengths of time. In this research, to estimate the parameters of the mineralized N and the rate of mineralization (k), double-pool kinetics equation was used. Mean while, the relationship between the parameters of N mineralization with nutrient uptake was investigated by conducting a pot experiment in a greenhouse. The results showed that the application of organic matter derived from groundnut produced higher mineralization. The N mineralization of soil that has been planted by cassava less than ten years was higher than it was on soil that has been planted by cassava more than 30 years. The total amount of N released on soil that has been planted by cassava less than 10 years reached 1086 mg kg-1. Whereas, the total amount of N released on soil already planted with cassava more than 30 years was 783 mg kg-1. Potential value of N (N0) and the mineralization rate coefficient (k) were higher in organic matter with a low ratio of C: N and on more fertile soil.

 
 
 
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Title:

Phytochemical screening and antityphoid properties of ethanolic leaf extracts of parquetina nigrescens

Author (s):

Akinyemi O. I. and Dada E. O.

Abstract:

The study carried out in vitro antibacterial activities of Parquetina nigrescens leaf extracts for the control of typhoid fever. Cold water extract, hot water extract and ethanolic extract of Parquetina nigrescens at various concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml, 250 mg/ml and 300mg/ml) were tested against Salmonella typhi at contact time 24, 48 and 72 hours respectively. All the concentrations showed varying zones of inhibition with ethanolic extract having the highest zone 10.5±0.57 mm at 300 mg/ml and contact time of 24 hours while the least zone of inhibition was recorded for cold water extract (2.9±0.63) at 50 mg/ml and contact time 72 hours. Of the ten standard antibiotics examined, Ciprofloxacin had the highest zone of growth inhibition 12.6±0.20mm. Tarivid had the least zones of inhibition 0.4±0.05 mm while Septrin and Sparloxacin had no effect on Salmonella typhi. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on S.typhi varied respectively with the ethanolic extract being the lowest values 12.5 mg/ml and 25 mg/ml. While the cold water extract had the highest values 50 mg/ml and 125 mg/ml respectively. Phytochemical screening of the P. nigrescens extracts showed the presence of saponins, alkaloids, tannins, anthraquinone, terpenoids, flavonoids, ascorbic acid and cardiac glycosides.

 
 
 
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Title:

Comparison of drip and sprinkler irrigation system for the cultivation plants vertically

Author (s):

Prayong Keeratiurai

Abstract:

Growing vegetables for own consumption in a household contributed to people living in the city could be more self-reliant in food factors. Studies in many countries have shown that urban farming could help bring food security, poverty reduction and promote a healthy mind and body healthy as well. This research study aims to evaluate the approach to drip and sprinkler irrigation of crops that were of cultivated plants vertically and relationship of water usage of both systems to yield per planted area. The vertical area was created that the fields were 4 floors in condo form to save space. On each floor could cultivation up to 10 square meters, it was divided into two parts which used different types of watering plants were drip irrigation system and sprinkler irrigation system. Sprinkler irrigation system has been watering using the amount of water as the program. The drip irrigation system reduced the water consumption to 50%. The drip irrigation system has major advantages that were watering high efficiency, use less water pressure, high yield, saving water. It provides higher crop yields when compared to the sprinkler irrigation system to the same of planting areas and quantity of water. The drip irrigation system could provide better performance than the sprinkler irrigation system. The drip irrigation was analyzed of economic value showed that PI values has greater than 1.0 and less than 2 year of payback period on the assessment of risk and depreciation for one year. The results also showed the analysis of IRR was 23.28% in the first year. This project was a worthwhile investment.

 
 
 
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Title:

Effects of drought stress on the alkaloid contents and growth parameters of catharanthus roseus

Author (s):

Mohammad Reza Amirjani

Abstract:

In the present investigation Catharanthus roseus, an important herb used in traditional as well as modern medicine, exposed to water deficit stress and possible changes in chlorophyll contents, photosynthesis rate, transpiration rate, growth parameters as well as total alkaloid content and vincristine and vinblastine levels were studied. Seedlings subjected to 4 different water-regimes. Experimental samples irrigated once every week or every second and third week and the control irrigated every day. Drought had adverse effect on height, weight and Relative water content of periwinkle. The photosynthetic activity and transpiration rate significantly decreased with increasing drought level. Total protein decreased to 77% and total chlorophyll decreased by 27%. Total alkaloid content significantly increased to maximum 187% compared to the control. Vincristine and vinblastine content of the seedlings grown under treatments3 increased to 175% and 171% compared to the control, respectively.

 
 
 
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Title: Production of brugmansia plants free of colombian datura virus by in vitro ribavirin chemotherapy
Author (s): Randall P. Niedz, Scott E. Hyndman, Daniel O. Chellemi and Scott Adkins
Abstract:

Brugmansia x candida Pers ‘Creamsickle’ plants produced by in vitro treatment with ribavirin, and no thermal therapy, remained polymerase chain reaction (PCR-) negative for Columbian datura virus (CDV) after one year. The plants were produced by establishing B. x candida ‘Creamsickle’ shoot cultures on autoclaved MS basal medium (Murashige and Skoog 1962), sucrose 30 g/L, myo-inositol 100 mg/L, thiamine HCl 1 mg/L, pyridoxine HCl 1 mg/L, nicotinic acid 1 mg/L, glycine 2 mg/L, BAP 1.1 μM, pH 5.7, and bacteriological agar (USB Corporation, Cleveland, Ohio, USA) 9.0 g/L with 15 ml of medium per 25×100 mm flat-bottomed glass culture tubes with polypropylene caps (Magenta Corporation, Chicago, Illinois, USA). The cultures were maintained in a growth room illuminated by cool-white fluorescent lamps (26 μmol m−2 s−1), constant 27 °C, and a 16h photoperiod. Four weeks after initiation, the cultures were transferred to the same medium in polypropylene capped glass tubes except that the BAP concentration was reduced to 0.5 μM. In vitro-derived shoots were excised and further dissected to 3-6 mm in length before transferring onto the same medium containing ribavirin at 0, 50, 87.5, or 100 mg/L; these shoots were cultured for 30 days. The ribavirin treated shoots were then transferred onto the multiplication medium without ribavirin for one subculture before being rooted in vitro on the same MS basal medium except with one half strength MS nitrogen salts and 3 μM IAA for four weeks followed by greenhouse acclimatization. In vitro-derived plants that expressed no CDV symptoms and tested PCR-negative one year after transfer to the greenhouse were produced over the entire range of 50-100 mg/L ribavirin tested. A single line, CS22B, from these PCR-negative plants was selected for long-term assessment - this line remains symptom-free and enzyme-linked immunosorbent assay-negative after 6 years.

 
 
 
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Title:

Decomposition and insect succession pattern of exposed domestic pig (Sus scrofa L.) carrion

Author (s): Rosina A. K. Kyerematen, Bernard A. Boateng, Michael Haruna and Vincent Y. Eziah
Abstract:

Pig carrion decomposition and insect succession patterns were monitored in the dry and wet seasons at the University of Ghana, Legon in the Greater Accra Region. The sequence and composition of the local carrion visiting fauna, as well as, the rate of decomposition of the carrion and their determinant climatic factors were measured. The complete decomposition of the carrion lasted 16 and 24 days for dry and wet season, respectively. Five stages of decomposition of the cadaver namely the fresh, bloated, active decay, advanced decay and dry remains were observed. In total, 19 species of insects from 14 families: Calliphoridae, Muscidae, Dolichopodidae, Gasterophilidae, Formicidae, Histeridae, Dermestidae, Cleridae, Lycidae, Staphilinidae, Pyrrhocoridae, Saturniidae and Therevidae were collected. Also a few species from the family Ixodidae (Arachnidae) were collected during the decomposition of the carrion. On account of their activity and frequency, the Calliphorid species, Lucilia rufifacies were the insects of greatest forensic importance. These blowflies were the early colonizers of the carrion in both seasons and remained throughout the decomposition process. Temperature, though, important in controlling decomposition rates of carrion, could not account for the differences in decomposition rates observed between the two seasons. Rainfall delayed colonization of carrion during both seasons and this might have played a major role in the delayed rate of degradation observed during the wet season. The succession patterns were typical for the seasonal periods and provide data on baseline fauna important for estimating postmortem interval in cases of human death in Ghana.

 
 
 
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